Quote:The film begins with the antagonist, Dr Heiter (Dieter Laser), kidnapping a truck driver by the side of a road. Later, two American tourists, Lindsay (Ashley C. Williams) and Jenny (Ashlynn Yennie), arrive at Heiter's house as they search for help after puncturing their car tire. Heiter drugs the girls and they awake along with the kidnapped trucker in a makeshift hospital ward in the cellar. Heiter informs the trucker that he is "not a match" and kills him. When the girls next wake, they are joined by a new captive, Japanese tourist Katsuro (Akihiro Kitamura).

The doctor explains that he is a world renowned expert at separating conjoined twins, but dreams of creating new beings that share a single digestive system by joining separate individuals via their mouths and anuses. He explains that his previous experiment, a "3-dog", died. However, he explains how he will attach the three human subjects to each other to create a "human centipede".